Chemical Synthesis and Expression of the HIV-1 Rev Protein
نویسندگان
چکیده
منابع مشابه
Chemical synthesis and expression of the HIV-1 Rev protein.
The HIV-1 Rev protein is responsible for shuttling partially spliced and unspliced viral mRNA out of the nucleus. This is a crucial step in the HIV-1 lifecycle, thus making Rev an attractive target for the design of anti-HIV drugs. Despite its importance, there is a lack of structural, biophysical, and quantitative information about Rev. This is mainly because of its tendency to undergo self-as...
متن کاملThe HIV-1 Rev protein.
The nuclear export of intron-containing HIV-1 RNA is critically dependent on the activity of Rev, a virally encoded sequence-specific RNA-binding protein. Rev shuttles between the nucleus and the cytoplasm and harbors both a nuclear localization signal and a nuclear export signal. These essential peptide motifs have now been shown to function by accessing cellular signal-mediated pathways for n...
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The HIV-1 protein Rev controls a critical step in viral replication by mediating the nuclear export of unspliced and singly-spliced viral mRNAs. Multiple Rev subunits assemble on the Rev Response Element (RRE), a structured region present in these RNAs, and direct their export through the Crm1 pathway. Rev-RRE assembly occurs via several Rev oligomerization and RNA-binding steps, but how these ...
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cloning, expression and library construction for hiv-1 tat protein
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ژورنال
عنوان ژورنال: ChemBioChem
سال: 2011
ISSN: 1439-4227
DOI: 10.1002/cbic.201100033